Implementation and Evaluation of Preimplantation Genetic Testing at Vilnius University Hospital Santaros Klinikos
نویسندگان
چکیده
Background and Objectives: The most effective treatment of infertility is in vitro fertilization (IVF). IVF with Preimplantation Genetic Testing (PGT) allows to identify embryos a genetic abnormality associated specific medical disorder select the optimal for transfer. PGT divided into structural rearrangement testing (PGT-SR), monogenetic (PGT-M), aneuploidy (PGT-A). This study mostly analyzes PGT-SR, also describes few cases PGT-M. aim this was implement procedure at Vilnius University Hospital Santaros Klinikos (VUHSK) Fertility Centre (SFC) perform retrospective analysis procedures after implementation.Materials Methods: A single-center carried out. population included infertile couples who underwent SFC, VUHSK from January 01st, 2017 December 31st, 2020. Ion PGM platform (Life Technologies, USA) ReproSeq PGS View Kit were used whole genome amplification. Results assessed using descriptive statistics.Results: successfully implemented 2017. During analyzed time period, thirty-four performed 26 couples. Two 2017, 7 – 2018, 13 2019, 12 In comparison all procedures, 2.5% PGT, highest percentage 2020 (3.8% procedures). main indication balanced chromosomal rearrangements (in 85.3% cases). 34 515 oocytes aspirated total, 309 fertilized, rate exceeded 60%. normal diploid karyotype found 46 (16.8%) biopsied embryos. Out 9 (26.5%) resulted clinical pregnancy. Six (66.7%) pregnancies confirmed 3 (33.3%) Three spontaneous abortion, 6 delivery.Conclusions: implementation successful. common reciprocal translocation. Oocytes 60%, less than one-fifth pregnancy achieved 2019 when almost half women conceived, which probably related experience gained by multidisciplinary team. first analyzing Lithuania, however, results should be interpreted caution due low number total performed.
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متن کاملPreimplantation Genetic Testing
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متن کاملPreimplantation Genetic Testing
Preimplantation genetic testing (PGT) involves analysis of biopsied cells as part of an assisted reproductive procedure. It is generally considered to be divided into 2 categories. Preimplantation genetic diagnosis (PGD) is used to detect a specific inherited disorder and aims to prevent the birth of affected children in couples at high risk of transmitting a disorder. Preimplantation genetic s...
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ژورنال
عنوان ژورنال: Acta medica Lituanica
سال: 2022
ISSN: ['1392-0138', '2029-4174']
DOI: https://doi.org/10.15388/amed.2022.29.2.9